Author: Kammer, Andreas R.; van der Burg, Sjoerd H.; Grabscheid, Benno; Hunziker, Isabelle P.; Kwappenberg, Kitty M.C.; Reichen, Jürg; Melief, Cornelis J.M.; Cerny, Andreas
Title: Molecular Mimicry of Human Cytochrome P450 by Hepatitis C Virus at the Level of Cytotoxic T Cell Recognition Document date: 1999_7_19
ID: 1vabzhs5_6
Snippet: Cell Lines. The EBV-transformed B cell line JY (HLA-A * 0201, -B7, -Cw7, -DR4, -Drw6, and -Dpw2) and K562 cells were cultured in RPMI 1640 medium supplemented with l -glutamine (2 mM), penicillin (50 U/ml), streptomycin (50 g/ml), and Hepes (5 mM) containing 10% (vol/vol) heat-inactivated FCS (FCSmedium). The human B lymphoblastoid cell line AHH-1 TK ϩրϪ derived from the RPMI 1788 cell line (HLA type: A2, Aw33, B7, B14) was maintained in FCS-m.....
Document: Cell Lines. The EBV-transformed B cell line JY (HLA-A * 0201, -B7, -Cw7, -DR4, -Drw6, and -Dpw2) and K562 cells were cultured in RPMI 1640 medium supplemented with l -glutamine (2 mM), penicillin (50 U/ml), streptomycin (50 g/ml), and Hepes (5 mM) containing 10% (vol/vol) heat-inactivated FCS (FCSmedium). The human B lymphoblastoid cell line AHH-1 TK ϩրϪ derived from the RPMI 1788 cell line (HLA type: A2, Aw33, B7, B14) was maintained in FCS-medium. H2A3 and h2D6 cells (AHH-1 TK ϩրϪ cells transfected with vectors coding for human CYP2A6 and CYP2D6, respectively; reference 25) were cultured in selective RPMI 1640 medium with 2 mM l -histidinol without l -histidine (Gentest Corp.) supplemented with l -glutamine (2 mM), penicillin (50 U/ml), streptomycin (50 g/ml), and Hepes (5 mM) containing 10% (vol/vol) heat-inactivated FCS (h2-medium). AHH-1 T/K ϩրϪ , h2A3, and h2D6 cells were a gift from Charles L. Crespi (Gentest Corp., Woburn, MA).
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