Author: Pipirou, Zoi; Powlesland, Alex S; Steffen, Imke; Pöhlmann, Stefan; Taylor, Maureen E; Drickamer, Kurt
Title: Mouse LSECtin as a model for a human Ebola virus receptor Document date: 2011_1_21
ID: 41i20yuy_33
Snippet: For competition binding assays and pH-dependence assays, polystyrene wells coated with CRD from LSECtin at a concentration of 50 µg mL −1 were probed with 125 I-labeled mannose-BSA or Ebola glycoprotein-Fc fusion protein (Mitchell et al. 2001) . For direct binding assays, biotin-tagged CRD was applied to streptavidin-coated wells that were incubated with unlabeled Ebola glycoprotein-Fc fusion protein at various dilutions followed alkaline phos.....
Document: For competition binding assays and pH-dependence assays, polystyrene wells coated with CRD from LSECtin at a concentration of 50 µg mL −1 were probed with 125 I-labeled mannose-BSA or Ebola glycoprotein-Fc fusion protein (Mitchell et al. 2001) . For direct binding assays, biotin-tagged CRD was applied to streptavidin-coated wells that were incubated with unlabeled Ebola glycoprotein-Fc fusion protein at various dilutions followed alkaline phosphatase-protein A conjugate and p-nitrophenylphosphate substrate (Powlesland et al. 2008) . Data were fitted to binding equations using the nonlinear least-squares fitting function of SigmaPlot (Systat Software, Hounslow, UK) to determine the half-maximal concentration for binding (K D ) or for inhibition of binding (K I ).
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