Multiplexed Nucleic Acid Programmable Protein Arrays

Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua

To demonstrate that the signal intensity for M-NAPPA was reproducible, we also tested the spot-to-spot, zone-to-zone and slide-to-slide variations by printing 80 gene plasmids on different locations across the M-NAPPA and NAPPA slides. Protein display was then examined with anti-GST antibody staining (Materials and Methods). The coefficient of variations (CVs) for spot-to-spot, zone-to-zone and slide-to-slide were 3.64±3.27%, 7.57±3.41% and 7.27±4.00% for M-NAPPA, respectively, and 7.63±10.58%, 12.13±7.56%% and 13.25±9.42%% for NAPPA, respectively (Table S4) .