Selected article for: "scale array and target discovery"

Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays
  • Document date: 2017_9_20
  • ID: 7t1o19kn_4
    Snippet: Of the cell-free approaches, NAPPA has achieved the highest densities with ~ 2,300 plasmids per slide where the distance between neighboring spots is 625 µm) and the cross-talk is less than 2%. However, cross-talk is increased when the feature spacing is reduced to 375 µm [21] . With ~ 2,300 plasmids per slide, five NAPPA slides are needed to screen a proteome-scale array with over 10,000 genes [18, 30] . Therefore, an increase in spot density .....
    Document: Of the cell-free approaches, NAPPA has achieved the highest densities with ~ 2,300 plasmids per slide where the distance between neighboring spots is 625 µm) and the cross-talk is less than 2%. However, cross-talk is increased when the feature spacing is reduced to 375 µm [21] . With ~ 2,300 plasmids per slide, five NAPPA slides are needed to screen a proteome-scale array with over 10,000 genes [18, 30] . Therefore, an increase in spot density would reduce the amount of labor, time, reagents, and cost needed for large-scale proteome analyses like target discovery and validation experiments.

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