Author: Daniels, Keith A.; Devora, Gene; Lai, Wayne C.; O'Donnell, Carey L.; Bennett, Michael; Welsh, Raymond M.
Title: Murine Cytomegalovirus Is Regulated by a Discrete Subset of Natural Killer Cells Reactive with Monoclonal Antibody to Ly49h Document date: 2001_7_2
ID: 6n3dmle6_10
Snippet: Depletion of NK Cells In Vivo. Mice were depleted of NK cells by a single intraperitoneal inoculation of 25-200 g of column-purified mAb 1F8 6-24 h before infection with virus. As controls, mice were inoculated with 100 l PBS containing 200 g of mAb 5E6, which recognizes Ly49 C/I, or with a dose of mAb to NK1.1 (PK136) titrated to eliminate LCMV-induced NK cell but not CTL activity (12) . Some control mice were inoculated with PBS which, as we sh.....
Document: Depletion of NK Cells In Vivo. Mice were depleted of NK cells by a single intraperitoneal inoculation of 25-200 g of column-purified mAb 1F8 6-24 h before infection with virus. As controls, mice were inoculated with 100 l PBS containing 200 g of mAb 5E6, which recognizes Ly49 C/I, or with a dose of mAb to NK1.1 (PK136) titrated to eliminate LCMV-induced NK cell but not CTL activity (12) . Some control mice were inoculated with PBS which, as we showed previously, results in MCMV titers indistinguishable from inoculations with mAb to Ly49A, D, G2, or C/I (22) .
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