Selected article for: "amplicon sequencing and relative bacterial abundance"
Author: Song, Charles; Chorath, Jeena; Pak, Youngju; Redjal, Nasser
Title: Use of Dipstick Assay and Rapid PCR-DNA Analysis of Nasal Secretions for Diagnosis of Bacterial Sinusitis in Children With Chronic Cough
  • Document date: 2019_1_7
    • ID: 5ykpnt89_19
    Snippet: This report, generated within 24 hours of sample collection, utilized the rapid quantitative PCR method using a Roche LightCycler 480 II instrument that detects 7 types of bacteria and the bacterial load, 1 fungus, 19 Resistance genes for the following antibiotics were included vancomycin, methicillins, beta-lactams, carbapenems, macrolides, aminoglycosides, tetracyclines, and quinolones. Once the presence of bacteria was identified, relative qua.....
    Document: This report, generated within 24 hours of sample collection, utilized the rapid quantitative PCR method using a Roche LightCycler 480 II instrument that detects 7 types of bacteria and the bacterial load, 1 fungus, 19 Resistance genes for the following antibiotics were included vancomycin, methicillins, beta-lactams, carbapenems, macrolides, aminoglycosides, tetracyclines, and quinolones. Once the presence of bacteria was identified, relative quantity (abundance) of these was reported in percentage of total bacterial load subsequently by using NGS (next-generation sequencing) machine. The method utilized universal 16S and internal transcribed space amplicon sequencing on the Ion Torrant Personal Genome Machine. A bacterium with abundance greater than 50% was considered to be a dominant species.

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