Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Document date: 1998_6_15
ID: 78fjem8s_22
Snippet: Samples were dissolved in SDS sample buffer and resolved by SDS-PAGE on 10 or 12% gels, transferred to nitrocellulose, and immunoblotted with an anti-HA polyclonal antibody and then with HRP-conjugated anti-rabbit IgG (Amersham Corp., Arlington Heights, IL). Detection was by enhanced chemiluminescence essentially as described by the manufacturer (Amersham Corp.). Dried gels of 35 S-labeled samples were scanned into a PhosphorImager workstation us.....
Document: Samples were dissolved in SDS sample buffer and resolved by SDS-PAGE on 10 or 12% gels, transferred to nitrocellulose, and immunoblotted with an anti-HA polyclonal antibody and then with HRP-conjugated anti-rabbit IgG (Amersham Corp., Arlington Heights, IL). Detection was by enhanced chemiluminescence essentially as described by the manufacturer (Amersham Corp.). Dried gels of 35 S-labeled samples were scanned into a PhosphorImager workstation using ImageQuant. HA band intensities were determined by summing the pixels in a constant volume rectangle.
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