Selected article for: "cell surface and ph low"
Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity
  • Document date: 1998_6_15
    • ID: 78fjem8s_46
    Snippet: Having confirmed that the single point mutant HA0s could be expressed at the cell surface as stable trimers that could be cleaved by trypsin, and having analyzed the ability of the mutant HAs to change conformation at low pH, we next tested the ability of cells expressing wt-or single point mutant HAs to fuse with RBCs. We assayed two different aspects of fusion: mixing of outer leaflet lipids and transfer of small aqueous contents. Figs. 6-11 di.....
    Document: Having confirmed that the single point mutant HA0s could be expressed at the cell surface as stable trimers that could be cleaved by trypsin, and having analyzed the ability of the mutant HAs to change conformation at low pH, we next tested the ability of cells expressing wt-or single point mutant HAs to fuse with RBCs. We assayed two different aspects of fusion: mixing of outer leaflet lipids and transfer of small aqueous contents. Figs. 6-11 display data for the Pro-substituted mutants.

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