Author: Firth, Andrew E.; Wills, Norma M.; Gesteland, Raymond F.; Atkins, John F.
Title: Stimulation of stop codon readthrough: frequent presence of an extended 3' RNA structural element Document date: 2011_4_27
ID: 2u49b7xo_14
Snippet: Tissue culture RT assays RT assays were performed using the dual luciferase reporter constructs, as previously described (62, 63) . To control for possible differences in stability of specific mRNA sequences, each RT construct was compared with a control construct that was identical except that the TGA stop codon was replaced with a TGG codon. RT efficiencies were calculated as (firefly activity/renilla activity) for the RT sequence normalized by.....
Document: Tissue culture RT assays RT assays were performed using the dual luciferase reporter constructs, as previously described (62, 63) . To control for possible differences in stability of specific mRNA sequences, each RT construct was compared with a control construct that was identical except that the TGA stop codon was replaced with a TGG codon. RT efficiencies were calculated as (firefly activity/renilla activity) for the RT sequence normalized by (firefly activity/renilla activity) for the corresponding TGG control sequence. Standard deviations were calculated based on six independent transfections.
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