Structural basis of development of multi-epitope vaccine against Middle East respiratory syndrome using in silico approach

Srivastava, Sukrit; Kamthania, Mohit; Singh, Soni; Saxena, Ajay K; Sharma, Nishi

ProtParam analysis showed that CTL MEVs have 500 amino acids, 50.4 kDa molecular weight, and 9.72 theoretical pI. The half-life in mammalian reticulocytes, yeast, and Escherichia coli was 30 hours, 20 hours, and 10 hours, respectively; aliphatic index was 61.68, and grand average of hydropathicity (GRAVY) was −0.020, indicating globular and hydrophilic nature of CTL MEVs; the instability index was 50.70, indicating that CTL MEVs are theoretically close to stable in nature. ProtParam study showed HTL MEV has 657 amino acids, 67.583 kDa molecular weight, and 9.29 theoretical pI. The half-life in mammalian reticulocytes, yeast, and E. coli was 30 hours, 20 hours, and 10 hours, respectively; aliphatic index was 94.22, and grand average of hydropathicity (GRAVY) was 0.337, both indicating globular and hydrophilic nature of HTL MEV; the instability index was 37.66, indicating theoretically stable nature of HTL MEV.