Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome Document date: 2013_3_26
ID: 1pbd4maf_10
Snippet: Before Pb 2+ ion-induced cleavage, RNA transcripts (10 pmol) were subjected to refolding as described earlier in the text. A freshly prepared solution of Pb(OAc) 2 was added to a final concentration of 0.5 mM, and the reaction mixtures were incubated for 10 min at room temperature (23) . Reactions were quenched by adding an equal volume of 20 mM EDTA followed by ethanol precipitation. Cleavage sites were detected by primer extension as described .....
Document: Before Pb 2+ ion-induced cleavage, RNA transcripts (10 pmol) were subjected to refolding as described earlier in the text. A freshly prepared solution of Pb(OAc) 2 was added to a final concentration of 0.5 mM, and the reaction mixtures were incubated for 10 min at room temperature (23) . Reactions were quenched by adding an equal volume of 20 mM EDTA followed by ethanol precipitation. Cleavage sites were detected by primer extension as described earlier in the text (19) .
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