Selected article for: "DENV replication and structure function relation"
Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome
  • Document date: 2013_3_26
    • ID: 1pbd4maf_33
    Snippet: Previous in vivo studies using DENV2 Renilla luciferase (Rluc) reporter replicon in BHK21 cells have shown that the 'flipping' mutation of PK1 severely reduced viral replication, whereas mutating both TL2 and PK1 to maintain their base pairing generated even more severe phenotype (8) . Also, introducing compensatory mutations in the 5 0 CS (mutant TL2 Flip PK1 Flip -3) to restore base pairing between the 5 0 -3 0 CS failed to improve replication,.....
    Document: Previous in vivo studies using DENV2 Renilla luciferase (Rluc) reporter replicon in BHK21 cells have shown that the 'flipping' mutation of PK1 severely reduced viral replication, whereas mutating both TL2 and PK1 to maintain their base pairing generated even more severe phenotype (8) . Also, introducing compensatory mutations in the 5 0 CS (mutant TL2 Flip PK1 Flip -3) to restore base pairing between the 5 0 -3 0 CS failed to improve replication, from which strong structure-and sequence-specific requirements for TL2 and PK1 function in DENV RNA replication have been proposed (8) . Consequently, we sought to investigate the structure-function relation of both regions in the context of DENV-MINI RNA.

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