Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus

Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub

Establishment and assembly of the ICA kit For establishment of ICA kit, the monoclonal antibody, anti-PED 3B12-1A6, purified from ascetic fluid using protein G resin (GE Healthcare) was coated onto a specific area (test line) of a nitrocellulose membrane, while goat antimouse IgG was coated onto another specific area (control line) on the same membrane. To produce the test conjugate, a different monoclonal antibody, anti-PED 1H12-1C6, was mixed with colloidal gold prepared by trisodium citrate reduction method as previously described, 12 and then the antibody mixture was treated by a previously described method. 12 The assay strips were prepared by assembly with the colloidal gold-conjugated glass fibre, the nitrocellulose membrane and an absorbent paper using polyvinyl chloride self-adhesive floor.