Title: The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal Document date: 1993_3_1
ID: 377v2ufn_5
Snippet: Expression constructs were made in plasmid pSVK3 (Pharmacia Fine Chemicals, Piscataway, NJ) which contains a multiple cloning site downstream from the SV-40 early promoter. Some eDNA sequences were generated by the PCR performed as described (Saiki et al., 1987) using the GeneAmp Kit (Perkin-Elmer Corp., Norwalk, CT). Parameters for PCR were denaturation at 94~ for 1 rain, annealing at 50~ for 2 min and extension at 72~ for 3 rain for 25 cycles. .....
Document: Expression constructs were made in plasmid pSVK3 (Pharmacia Fine Chemicals, Piscataway, NJ) which contains a multiple cloning site downstream from the SV-40 early promoter. Some eDNA sequences were generated by the PCR performed as described (Saiki et al., 1987) using the GeneAmp Kit (Perkin-Elmer Corp., Norwalk, CT). Parameters for PCR were denaturation at 94~ for 1 rain, annealing at 50~ for 2 min and extension at 72~ for 3 rain for 25 cycles. Unless otherwise indicated, standard methods (Sambrook et al., 1989) were used for DNA purification, restriction endonuclease digestion, ligation, transformation, and preparation of plasmid DNA.
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