Selected article for: "master mix and Sybr Green master mix"
Author: Li, Xinyu; Huang, Lei; Gao, Weijie
Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)
  • Document date: 2019_1_27
    • ID: 037d5r9i_13
    Snippet: Total RNA was extracted by Trizol reagent and reverse transcription was conducted by using Superscript III RT (Invitrogen) and random primers. Real-time PCR was performed with SYBR Green PCR Master Mix (Applied TaKaRa, Otsu, Shiga, Japan). The nucleotide sequences of the primers were as follows: GAPDH, 5'-ATGGGGAAGGTGAAGGTCG-3' and 5'-GGGGTCATTGATGGCAACAATA-3'; TRIM55, 5'-GGTTTT GGATAGACATGGGGT-3', and 5'-TTCTCCTCTTGGGTTCGGGT-3'. The results were.....
    Document: Total RNA was extracted by Trizol reagent and reverse transcription was conducted by using Superscript III RT (Invitrogen) and random primers. Real-time PCR was performed with SYBR Green PCR Master Mix (Applied TaKaRa, Otsu, Shiga, Japan). The nucleotide sequences of the primers were as follows: GAPDH, 5'-ATGGGGAAGGTGAAGGTCG-3' and 5'-GGGGTCATTGATGGCAACAATA-3'; TRIM55, 5'-GGTTTT GGATAGACATGGGGT-3', and 5'-TTCTCCTCTTGGGTTCGGGT-3'. The results were analyzed by 2 -DDCT method with GAPDH as the internal control. Each sample was analyzed in triplicate.

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