Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_30
Snippet: A virus infection inhibition assay was performed to evaluate whether the infective ability of the pseudoviruses could be inhibited by an anti-RVFV antibody. As shown in Fig. 6 , after incubation with a polyclonal antibody against the RVFV protein, the RVFV pseudoviruses could not effectively infect 293T cells, whereas the VSV pseudoviruses and the polyclonal antibody against the RVFV protein could not effectively defend against virus infection in.....
Document: A virus infection inhibition assay was performed to evaluate whether the infective ability of the pseudoviruses could be inhibited by an anti-RVFV antibody. As shown in Fig. 6 , after incubation with a polyclonal antibody against the RVFV protein, the RVFV pseudoviruses could not effectively infect 293T cells, whereas the VSV pseudoviruses and the polyclonal antibody against the RVFV protein could not effectively defend against virus infection in 293T cells. Therefore, the antibody inhibition assay indicated that the infective ability of the RVFV pseudoviruses could be inhibited by specific antibodies. These results further indicated that the obtained RVFV pseudoviruses could bind to specific antibodies and could be used as the antigen in a neutralization assay; thus, the pseudoviruses could be effectively applied in an antibody neutralization assay.
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