Proceedings of the Food and Drug Administration public workshop on pathogen reduction technologies for blood safety 2018 (Commentary, p. 3026)

Atreya, Chintamani; Glynn, Simone; Busch, Michael; Kleinman, Steve; Snyder, Edward; Rutter, Sara; AuBuchon, James; Flegel, Willy; Reeve, David; Devine, Dana; Cohn, Claudia; Custer, Brian; Goodrich, Raymond; Benjamin, Richard J.; Razatos, Anna; Cancelas, Jose; Wagner, Stephen; Maclean, Michelle; Gelderman, Monique; Cap, Andrew; Ness, Paul

As with all PRTs, a crucial question that must be answered is whether antimicrobial efficacy can be imparted with negligible and/or minimal effect on the quality of the blood component itself. Results of sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Western blotting analysis were presented which highlighted the potential for "safe" treatment of blood plasma and the need for determining upper and lower threshold treatment levels, which would facilitate decontamination while retaining plasma and PLT integrity. With regard to PLTs, an evaluation of the in vivo recovery of light-treated (10 mW/cm 2 for 8 hr) PLTs in a murine model demonstrated no difference to that of nontreated PLTs. These results are encouraging; however, further investigation is needed on the impact of dose delivery (high irradiance light delivered over rapid exposure periods compared to low irradiance light over longer exposures), as this will influence the optimal treatment conditions for the blood components. Discussions highlighted the potential for the technology to be utilized for rapid treatment before storage (for PLTs or plasma) or incorporation into standard storage conditions for PLTs with the aim of extending the shelf life of these sensitive components.