Author: Liu, Hong Yan; Gao, Xiaohu
Title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras Document date: 2013_11_7
ID: 0atfsivf_19
Snippet: Materials. Vendors for specific chemicals are listed below. In general, restriction enzymes were obtained from New England BioLabs, and cell culture products were purchased from Gibco/Invitrogen. Chimera composed of aptamer targeting PSMA and siRNA targeting GFP. ssDNA of the PSMA aptamer (39 nucleotides, 59-GGGAGGACGATGCGGATCA-GCCATGTTTACGTCACTCCT-39) was chemically synthesized by Integrated DNA Technologies (IDT) and used as the template to gen.....
Document: Materials. Vendors for specific chemicals are listed below. In general, restriction enzymes were obtained from New England BioLabs, and cell culture products were purchased from Gibco/Invitrogen. Chimera composed of aptamer targeting PSMA and siRNA targeting GFP. ssDNA of the PSMA aptamer (39 nucleotides, 59-GGGAGGACGATGCGGATCA-GCCATGTTTACGTCACTCCT-39) was chemically synthesized by Integrated DNA Technologies (IDT) and used as the template to generate one strand of the siRNA-aptamer chimera. For amplification, PCR was performed with 39 primer containing the anti-sense strand of GFP siRNA (underlined) and 59 primer containing T7 RNA polymerase promoter site (bolded). The PCR primer sequences are:
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