Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Both RNA and DNA viruses target the nucleolus and its structure, and the proteome can change in response to viral infection (61, 106) . In this study, the quantitative proteomic analysis indicated that two of the most common and most studied nucleolar proteins, nucleolin and nucleophosmin, were ablated in HRSV-infected cells. For nucleolin, this was confirmed by Western blot analysis (Fig. 11) , demonstrating the strength of the SILAC approach. Recently, it has been shown that lamin B1 interacts with nucleophosmin to maintain nucleolar structure for ribosome biogenesis (107) . However, the loss of lamin B1 resulted in the breakdown of the nucleolus (107) . Our analysis indicated that lamin B1 was potentially cleaved and depleted in HRSV-infected cells (Fig. 1C) . However, the observation of degradation of lamin B was antibody-dependent (e.g. compare Fig. 1C with Fig. 11 ). Nucleolin was also observed to decrease in human metapneumovirus-infected cells using 2DE (27) .