Alteration of somatostatin receptor 2 expression in canine mammary gland tumor

SAKAI, Kosei; YONEZAWA, Tomohiro; YAMAWAKI, Hideyuki; OYAMADA, Toshifumi

All experiments were approved by the Committees on the Ethics of Animal Experiments of Kitasato University and the University of Tokyo. For immunohistochemistry, 48 samples of canine mammary gland tissue embedded in paraffin blocks were obtained from the histopathological files in the Veterinary Pathology Laboratory, Kitasato University. Although the classification method about simple carcinoma is under controversy [18] , the diagnosis of all specimens was based on the WHO classification [11] . Immunohistochemical staining was performed using the rabbit polyclonal antibodies against human SSTR2 (1:300 dilution, bs-1138R, Bioss, Boston, MA, U.S.A.). Peroxidase-conjugated anti-rabbit immunoglobulin G (Histofine Simple Stain MAX-PO (R), Nichirei, Tokyo, Japan) was used as the secondary antibody. After immunoreaction, sections were colorized with diaminobenzidine and counterstained with hematoxylin. Canine pancreas tissues were used as positive controls. Negative controls were carried out by replacing the primary antibodies with antibody diluents.