Selected article for: "blot analysis and northern blot analysis"
Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits
  • Document date: 2015_3_11
    • ID: 64cnoqpi_60
    Snippet: Expression of the Fluc was reduced for all reporter plasmids co-expressed with the scAluY NF1 RNA ( Figure 6C) . Similarly, the cap-dependent translation of Rluc from the upstream open reading frame of the bicistronic mRNAs was also diminished ( Figure 6C ). In contrast, the negative controls, 4.5S and 7SL RNAs, did not significantly affect luciferase expression, although higher levels of 7SL RNA had a slight tendency to increase it. Northern blo.....
    Document: Expression of the Fluc was reduced for all reporter plasmids co-expressed with the scAluY NF1 RNA ( Figure 6C) . Similarly, the cap-dependent translation of Rluc from the upstream open reading frame of the bicistronic mRNAs was also diminished ( Figure 6C ). In contrast, the negative controls, 4.5S and 7SL RNAs, did not significantly affect luciferase expression, although higher levels of 7SL RNA had a slight tendency to increase it. Northern blot analysis revealed that each reporter plasmid produced an mRNA of the expected size, which was present in comparable amounts in the samples expressing ncRNAs (Supplementary Figure S8A) .

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