Author: Larouche-Lebel, Éva; Loughran, Kerry A.; Oyama, Mark A.; Solter, Phil F.; Laughlin, Danielle S.; Sánchez, Melissa D.; Assenmacher, Charles-Antoine; Fox, Philip R.; Fries, Ryan C.
Title: Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease Document date: 2019_6_28
ID: 6ek80l79_3
Snippet: Validation of the ACE2 assay included running internal controls with a kit-supplied ACE2 positive control and a synthetic peptide that inhibits ACE2 activity, which serves as a negative control. The ACE2 inhibitor is mixed in separate reaction wells with the positive control and the test samples to confirm that substrate degradation over the 120 min is due to ACE2 activity......
Document: Validation of the ACE2 assay included running internal controls with a kit-supplied ACE2 positive control and a synthetic peptide that inhibits ACE2 activity, which serves as a negative control. The ACE2 inhibitor is mixed in separate reaction wells with the positive control and the test samples to confirm that substrate degradation over the 120 min is due to ACE2 activity.
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