Author: Wilfred, Eneku; Mutebi, Francis; Mwiine, Frank Norbert; James, Okwee-Acai; Lonzy, Ojok
Title: Porcine Circovirus type 2 – Systemic disease on pig farms and associated knowledge of key players in the pig industry in Central Uganda Document date: 2018_8_28
ID: 6wkxhrwl_18
Snippet: For PCR, 10 sections (only spleen and lymph nodes) of 10 µm thick for each pig were cut and placed in 1.5 mL eppendorf® tubes containing 1 mL of xylene for deparaffinization. In between samples, microtome blades were cleaned with xylene to prevent cross contamination of samples. Extraction of DNA was done using QIAamp® DNA Formalin Fixed Paraffin Embedded (FFPE) Tissue Kit (QIAGEN GmbH, D-40724 Hilden, Germany) as per the manufacturer's protoc.....
Document: For PCR, 10 sections (only spleen and lymph nodes) of 10 µm thick for each pig were cut and placed in 1.5 mL eppendorf® tubes containing 1 mL of xylene for deparaffinization. In between samples, microtome blades were cleaned with xylene to prevent cross contamination of samples. Extraction of DNA was done using QIAamp® DNA Formalin Fixed Paraffin Embedded (FFPE) Tissue Kit (QIAGEN GmbH, D-40724 Hilden, Germany) as per the manufacturer's protocol. Negative and positive controls from the University of Giessen, Germany were also processed alongside the samples to validate the findings.
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