A review of vaccine development and research for industry animals in Korea

Lee, Nak-Hyung; Lee, Jung-Ah; Park, Seung-Yong; Song, Chang-Seon; Choi, In-Soo; Lee, Joong-Bok

With rich information available on viral genomes along with the advancement of available genetic tools, scientists are able to deliberately manipulate viral genetic material in order to create attenuated live or inactivated viruses. One of the great advancements in this field is the ability to create an infectious clone that contains a full viral genome sequence on a bacterial plasmid. The viral genes on the infectious clone can be easily manipulated and transfected into susceptible cells in order to engineer genetically manipulated virus. With this technique, a chimeric virus shuffled with different viral genomes can be generated for the desired outcome. However, there is a concern that such virus may become a recombinant with field strains and consequently pathogenicity may be restored.