Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_37
Snippet: None of the Alu RNPs affected the association of [ 35 S]-Met-tRNA i Met with the 40S subunit ( Figure 3A ). In contrast, a significant reduction in [ 32 P]-labeled mRNA was found to comigrate with 40S subunits in the presence of AluY A and scAluY A RNPs and a corresponding increase of free RNA was observed in the upper fractions ( Figure 3B ). As also observed in the toeprint assay ( Figure 1E ), the effect was more pronounced for the AluY A RNP .....
Document: None of the Alu RNPs affected the association of [ 35 S]-Met-tRNA i Met with the 40S subunit ( Figure 3A ). In contrast, a significant reduction in [ 32 P]-labeled mRNA was found to comigrate with 40S subunits in the presence of AluY A and scAluY A RNPs and a corresponding increase of free RNA was observed in the upper fractions ( Figure 3B ). As also observed in the toeprint assay ( Figure 1E ), the effect was more pronounced for the AluY A RNP than for the scAluY A RNP. Addition of the inactive SA110 RNP to the assembly reaction did not affect the association of the mRNA with the 40S subunit.
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