Author: Lin, Tsai-Yu; Chin, Christopher R.; Everitt, Aaron R.; Clare, Simon; Perreira, Jill M.; Savidis, George; Aker, Aaron M.; John, Sinu P.; Sarlah, David; Carreira, Erick M.; Elledge, Stephen J.; Kellam, Paul; Brass, Abraham L.
Title: Amphotericin B Increases Influenza A Virus Infection by Preventing IFITM3-Mediated Restriction Document date: 2013_11_21
ID: 10ynhrl3_33
Snippet: Cells were plated on glass-bottom 6c m dishes (MatTek) 16 hr prior to analysis. Cells were pretreated with fresh media or fresh media containing 1 mM of AmphoB for 1 hr then stained with Vibrant DiI or DiO (Invitrogen) for 15 min at 37 C, then washed with 13 PBS twice. FRAP was then done on the cells using a Leica SP5 with the Leica FRAP wizard. At least ten cells were measured per experiment, and LAS AF Lite (Leica) software was used to quantify.....
Document: Cells were plated on glass-bottom 6c m dishes (MatTek) 16 hr prior to analysis. Cells were pretreated with fresh media or fresh media containing 1 mM of AmphoB for 1 hr then stained with Vibrant DiI or DiO (Invitrogen) for 15 min at 37 C, then washed with 13 PBS twice. FRAP was then done on the cells using a Leica SP5 with the Leica FRAP wizard. At least ten cells were measured per experiment, and LAS AF Lite (Leica) software was used to quantify the intensity of the photobleached area, the total cell fluorescence, and an area outside of the cells for normalization. The normalized intensities of the photobleached areas were then averaged together to form a representative curve of each condition.
Search related documents:
Co phrase search for related documents- cell fluorescence and Leica software: 1, 2
- cell fluorescence and Lite Leica software: 1, 2
- cell fluorescence and photobleached area: 1
- cell fluorescence and total cell fluorescence: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date