Selected article for: "cycle threshold and rrt pcr testing"
Author: Kim, Mi-Na; Ko, Young Jin; Seong, Moon-Woo; Kim, Jae-Seok; Shin, Bo-Moon; Sung, Heungsup
Title: Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR
  • Document date: 2016_6_24
    • ID: 1le537yd_5
    Snippet: To evaluate the clinical sensitivity of the assays, 18 specimens from the lower respiratory tract, including 14 known positive specimens, were obtained from five institutions. The RNA eluates of nine specimens that remained after initial clinical MERS-CoV rRT-PCR testing were used following storage of < 2 months at -70°C at each institution. The other nine specimens were manually prepared in our laboratory without any pretreatment and were found.....
    Document: To evaluate the clinical sensitivity of the assays, 18 specimens from the lower respiratory tract, including 14 known positive specimens, were obtained from five institutions. The RNA eluates of nine specimens that remained after initial clinical MERS-CoV rRT-PCR testing were used following storage of < 2 months at -70°C at each institution. The other nine specimens were manually prepared in our laboratory without any pretreatment and were found to be inadequate for evaluating clinical sensitivity owing to the extremely high levels of PCR inhibition. Therefore, only the first nine specimens were used to evaluate clinical sensitivity, and the other nine specimens with high inhibition were used to evaluate the effects of PCR inhibition on positive results. In addition to determining the numbers of positives and negatives, the cycle threshold (Ct) values of the target genes and internal control were analyzed.

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