Author: Hu, Zhiqiang; Xing, Yaling; Qian, Yuanyu; Chen, Xiaojuan; Tu, Jian; Ren, Lening; Wang, Kai; Chen, Zhongbin
Title: Anti-radiation damage effect of polyethylenimine as a toll-like receptor 5 targeted agonist Document date: 2012_10_26
ID: 6fp8nly0_12
Snippet: To evaluate PEI cytotoxicity, cell viability was examined by MTT assay. Briefly, HEK 293T cells were seeded in 96-well plates at a density of 8 × 10 3 for 24 h. When cells were 40-60% confluent, the cells were incubated at various concentrations of PEI in DMEM for 48 h. Ten microliters of MTT was added to each well and incubated for 4 h at 37°C. One hundred microliters of formanzan lysis was then added to each well and incubated for another 4 h.....
Document: To evaluate PEI cytotoxicity, cell viability was examined by MTT assay. Briefly, HEK 293T cells were seeded in 96-well plates at a density of 8 × 10 3 for 24 h. When cells were 40-60% confluent, the cells were incubated at various concentrations of PEI in DMEM for 48 h. Ten microliters of MTT was added to each well and incubated for 4 h at 37°C. One hundred microliters of formanzan lysis was then added to each well and incubated for another 4 h at 37°C. The absorbance of the solubilized formanzan was measured at 570 nm. The viability of cells incubated in control medium was considered as 100%.
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