Selected article for: "address limitation and low sensitivity"
Author: Hayden C. Metsky; Katherine J. Siddle; Adrianne Gladden-Young; James Qu; David K. Yang; Patrick Brehio; Andrew Goldfarb; Anne Piantadosi; Shirlee Wohl; Amber Carter; Aaron E. Lin; Kayla G. Barnes; Damien C. Tully; Björn Corleis; Scott Hennigan; Giselle Barbosa-Lima; Yasmine R. Vieira; Lauren M. Paul; Amanda L. Tan; Kimberly F. Garcia; Leda A. Parham; Ikponmwonsa Odia; Philomena Eromon; Onikepe A. Folarin; Augustine Goba; Etienne Simon-Lorière; Lisa Hensley; Angel Balmaseda; Eva Harris; Douglas Kwon; Todd M. Allen; Jonathan A. Runstadler; Sandra Smole; Fernando A. Bozza; Thiago M. L. Souza; Sharon Isern; Scott F. Michael; Ivette Lorenzana; Lee Gehrke; Irene Bosch; Gregory Ebel; Donald Grant; Christian Happi; Daniel J. Park; Andreas Gnirke; Pardis C. Sabeti; Christian B. Matranga
Title: Capturing diverse microbial sequence with comprehensive and scalable probe design
  • Document date: 2018_3_12
    • ID: a9lkhayg_35
    Snippet: In recent years metagenomic sequencing has been widely used to investigate viral infections and outbreaks, but is often limited in practice due to low sensitivity. Capture using oligonucleotide probes to enrich viral content is one approach that can address this limitation [13] [14] [15] [23] [24] [25] . Here we describe CATCH, a method that condenses highly diverse target sequence data into a small number of oligonucleotides, enabling more effic.....
    Document: In recent years metagenomic sequencing has been widely used to investigate viral infections and outbreaks, but is often limited in practice due to low sensitivity. Capture using oligonucleotide probes to enrich viral content is one approach that can address this limitation [13] [14] [15] [23] [24] [25] . Here we describe CATCH, a method that condenses highly diverse target sequence data into a small number of oligonucleotides, enabling more efficient and sensitive sequencing that is only biased by the extent of known diversity. We show that capture with probe sets designed by CATCH improved viral genome detection and recovery, across a range of sample source materials, while accurately preserving sample complexity of the targets. Probe sets we present here have also helped us to assemble genomes of low titer viruses in other patient samples: V ZC for suspected ZIKV cases 7 and V ALL for improving rapid detection of Powassan virus in a clinical case 52 .

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