Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection Document date: 2018_2_15
ID: 1xjmlwqr_85
Snippet: Through using a mixture of AuNPs and silver NPs (AgNPs), a colorimetric assay has been established for the multiplex, one-pot molecular detection of KSHV and its frequently confounding disease bacillary angiomatosis [57] . In this assay, two types of probes are prepared from AuNPs and AgNPs separately functionalized with KSHV and Bartonellaspecific DNA-capturing DNA probes, respectively, and mixed with the target DNA. Then, the color of the react.....
Document: Through using a mixture of AuNPs and silver NPs (AgNPs), a colorimetric assay has been established for the multiplex, one-pot molecular detection of KSHV and its frequently confounding disease bacillary angiomatosis [57] . In this assay, two types of probes are prepared from AuNPs and AgNPs separately functionalized with KSHV and Bartonellaspecific DNA-capturing DNA probes, respectively, and mixed with the target DNA. Then, the color of the reaction mixture changes in a manner dependent on the type of target DNA added to the reaction solution: the addition of KSHV DNA causes AuNP conjugates to aggregate and the solution to appear murky yellow-orange, i.e., the specific color of the nonaggregated AgNPs, while the addition of Bartonella DNA causes AgNP conjugates to aggregate and the solution to appear pink, i.e., the color of nonaggregated AuNPs (Fig. 11C ). In addition to being simple, this multicolor-change system successfully allows the multiplex detection of targets present in nanomolar concentrations.
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