Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_30
Snippet: Cell death, either necrosis or apoptosis, was assayed by addition of 50 l of a second luminogenic cell-impermeant peptide AAF-aminoluciferin substrate, and then analyzed on a luminescence plate reader. The AAF-aminoluciferin is a peptide substrate for cellular proteases which are released from compromised cells (11) . Released proteases liberate aminoluciferin that is measured as luminescence generated by a recombinant Luciferase, whereas uncleav.....
Document: Cell death, either necrosis or apoptosis, was assayed by addition of 50 l of a second luminogenic cell-impermeant peptide AAF-aminoluciferin substrate, and then analyzed on a luminescence plate reader. The AAF-aminoluciferin is a peptide substrate for cellular proteases which are released from compromised cells (11) . Released proteases liberate aminoluciferin that is measured as luminescence generated by a recombinant Luciferase, whereas uncleaved AAFaminoluciferin is not a substrate for recombinant luciferase, so viable cells generate a modest luminescence background. The live/dead cell ratio was estimated as the GF-AFC to AAF-aminoluciferin signals.
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