Selected article for: "CMV promoter enhancer and human CMV promoter enhancer"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_39
    Snippet: To investigate whether the post-translational maturation of proteins produced with the C3P3-G1 expression system in CHO-K1 cells was altered, the functional activity of the human EPO (hEPO) glycoprotein produced with the C3P3-G1 system was assessed. CHO-K1 cells were plated in 24well plates at a density of 1 × 10 5 cells per well. To avoid cross-contamination by the cytokines present in the fetal calf serum, CHO-K1 cells were cultured in Panseri.....
    Document: To investigate whether the post-translational maturation of proteins produced with the C3P3-G1 expression system in CHO-K1 cells was altered, the functional activity of the human EPO (hEPO) glycoprotein produced with the C3P3-G1 system was assessed. CHO-K1 cells were plated in 24well plates at a density of 1 × 10 5 cells per well. To avoid cross-contamination by the cytokines present in the fetal calf serum, CHO-K1 cells were cultured in Panserin X10 serum-free medium. Cells were cotransfected with pCMV-NP868R-(G 4 S) 2 -K1ERNAP(R551S) and pK1E(G)-hEPO plasmids (encoding for the wild-type human erythropoietin precursor gene under control of the K1E phage promoter) using the Lipofectamine 2000 reagent. As a comparator, cells also transfected with the pCMVScript-hEPO plasmid (ORF of the wild-type hEPO precursor under control of the human IE1 CMV promoter/enhancer). Culture medium from CHO-K1 cells was removed on the second day after transfection, and erythropoietin titers were assessed by ELISA as described above.

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