Selected article for: "enhancer gene promoter and gene promoter"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_42
    Snippet: The kinetics of mRNA synthesis by the C3P3-G1 expression system was assessed by quantitative RT-PCR. HEK-293 cells were plated in 24-well plates at a density of 1 × 10 5 cells per well and transfected with either pCMV-NP868R-(G 4 S) 2 -K1ERNAP(R551S) plus the pK1E(G)-Luciferase plasmid, or the pCMVScript-Luciferase plasmid (Luciferase gene under control of the human CMV promoter/enhancer) using the Lipofectamine 2000 reagent......
    Document: The kinetics of mRNA synthesis by the C3P3-G1 expression system was assessed by quantitative RT-PCR. HEK-293 cells were plated in 24-well plates at a density of 1 × 10 5 cells per well and transfected with either pCMV-NP868R-(G 4 S) 2 -K1ERNAP(R551S) plus the pK1E(G)-Luciferase plasmid, or the pCMVScript-Luciferase plasmid (Luciferase gene under control of the human CMV promoter/enhancer) using the Lipofectamine 2000 reagent.

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