Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_57
Snippet: In contrast to the two-subunit vaccinia virus capping enzyme, some viruses and episomes encode single-subunit capping enzymes. With the rationale that single-subunit capping enzymes would be simpler to use in routine expression systems, we next tested the activity of distinct singlesubunit capping enzymes. Subsequently, artificial enzymes, collectively designated as C3P3-G1 enzymes, were generated by in-frame fusion of candidate capping enzymes t.....
Document: In contrast to the two-subunit vaccinia virus capping enzyme, some viruses and episomes encode single-subunit capping enzymes. With the rationale that single-subunit capping enzymes would be simpler to use in routine expression systems, we next tested the activity of distinct singlesubunit capping enzymes. Subsequently, artificial enzymes, collectively designated as C3P3-G1 enzymes, were generated by in-frame fusion of candidate capping enzymes to the amino-terminal end of T7RNAP via (G 4 S) 4 linkers ( Figure 3A ).
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