Selected article for: "gene sequence and genomic sequence"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_7
    Snippet: Plasmids containing the Firefly Luciferase gene were used to optimize C3P3-G1 enzymes and DNA template sequences. They consist of a phage RNA polymerase promoter, variable 5 -UTR, Kozak consensus sequence followed by the ORF of the Luciferase gene from Photinus pyralis and stop codon, variable 3 -UTR, poly [A] track that was routinely of 40 adenosine residues, followed by a selfcleavage RNA sequence that was generally the genomic ribozyme sequenc.....
    Document: Plasmids containing the Firefly Luciferase gene were used to optimize C3P3-G1 enzymes and DNA template sequences. They consist of a phage RNA polymerase promoter, variable 5 -UTR, Kozak consensus sequence followed by the ORF of the Luciferase gene from Photinus pyralis and stop codon, variable 3 -UTR, poly [A] track that was routinely of 40 adenosine residues, followed by a selfcleavage RNA sequence that was generally the genomic ribozyme sequence from the hepatitis D virus, and terminated by the bacteriophage T7 10 transcription stop. Restriction enzymatic sites were inserted between each motif of the luciferase plasmids to allow easy swapping of each motif by subcloning. The plasmids are identified by the corresponding ORF (e.g. Luciferase) preceded by the phage promoter (e.g. pT710-Luciferase).

    Search related documents:
    Co phrase search for related documents
    • Luciferase gene and phage promoter: 1, 2
    • Luciferase gene and polymerase promoter: 1, 2
    • Luciferase gene and RNA polymerase promoter: 1
    • Luciferase gene and RNA sequence: 1, 2, 3, 4
    • luciferase plasmid and phage promoter: 1
    • luciferase plasmid and polymerase promoter: 1, 2
    • luciferase plasmid and RNA polymerase promoter: 1
    • luciferase plasmid and RNA sequence: 1
    • phage promoter and polymerase promoter: 1
    • phage promoter and RNA polymerase promoter: 1
    • phage promoter and RNA sequence: 1
    • polymerase promoter and RNA polymerase promoter: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47
    • polymerase promoter and RNA sequence: 1, 2, 3, 4, 5, 6, 7, 8
    • RNA sequence and template sequence: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25