Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_84
Snippet: Besides its therapeutics uses, the C3P3 expression system should be also considered for its applications in cellulo. For instance, C3P3 expression system can be also used for transient expression studies for phenotypic screening or others. However, the artificial nature of C3P3-G1 system makes it inappropriate for investigation of host-cell transcriptional or translational regulation. In addition, the high performances of the C3P3-G1 system in CH.....
Document: Besides its therapeutics uses, the C3P3 expression system should be also considered for its applications in cellulo. For instance, C3P3 expression system can be also used for transient expression studies for phenotypic screening or others. However, the artificial nature of C3P3-G1 system makes it inappropriate for investigation of host-cell transcriptional or translational regulation. In addition, the high performances of the C3P3-G1 system in CHO-K1 cells and the lack of detectable cell toxicity of the C3P3 enzyme suggest that stable C3P3 cell lines could be generated for production of recombinant proteins.
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