Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species

Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB

For Cockatiel samples, the mean signal intensity of 21,032 was obtained for positive Cockatiel plasma tested using the anti-macaw IgY secondary antibody was significantly greater (P<0.05) than the signal intensity obtained when using any of the other three secondary antibodies. The signal intensity of the positive plasma dots obtained for positive Cockatiel plasma was significantly greater (P<0.05) than that obtained for negative Cockatiel plasma when using anti-macaw or anti-bird IgY secondary antibody. The signal intensity values did not significantly differ between the positive and negative samples when anti-chicken or anti-duck IgY secondary antibodies were used. For Monk parakeet samples, the mean signal intensity of 17,785 obtained for the positive plasma tested using anti-macaw IgY secondary antibody was significantly greater (P<0.05) than the signal intensity when the other three secondary antibodies were used. The signal intensity of the positive plasma was significantly greater (P<0.05) than that of the negative plasma when using anti-macaw or anti-bird IgY secondary antibody. The signal intensity values did not differ significantly between the positive and negative samples when anti-chicken or anti-duck IgY secondary antibodies were used.