Author: Avirutnan, Panisadee; Hauhart, Richard E.; Marovich, Mary A.; Garred, Peter; Atkinson, John P.; Diamond, Michael S.
Title: Complement-Mediated Neutralization of Dengue Virus Requires Mannose-Binding Lectin Document date: 2011_12_13
ID: 1x3n5job_19
Snippet: During natural DENV infection, following the bite of an infected mosquito, the human host first encounters insect cellgenerated virus. Subsequent rounds of infection produce virus from human cells. In contrast to that observed with WNV (25), MBL in serum neutralized both insect cell-and mammalian cellderived DENV, suggesting a role for MBL in controlling both stages of DENV infection. MBL recognizes adjacent equatorial monosaccharide hydroxyl gro.....
Document: During natural DENV infection, following the bite of an infected mosquito, the human host first encounters insect cellgenerated virus. Subsequent rounds of infection produce virus from human cells. In contrast to that observed with WNV (25), MBL in serum neutralized both insect cell-and mammalian cellderived DENV, suggesting a role for MBL in controlling both stages of DENV infection. MBL recognizes adjacent equatorial monosaccharide hydroxyl groups that are present on mannose, N-acetylglucosamine (GlcNAc), and fucose, which are commonly found on microorganisms (14) . More efficient neutralization of DENV (both insect cell and mammalian cell derived) compared to neutralization of WNV (only insect cell derived) by MBL may be due to the additional N-linked glycan at Asn-67, which confers binding to the attachment factor DC-SIGN (5) and is unique to DENV among flaviviruses. The results of our experiments also established that MBL can bind and neutralize insect cell-derived DENV directly in the absence of further complement activation; this did not occur with mammalian cell-derived DENV.
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