Selected article for: "ATP hydrolysis and duplex unwinding"
Author: Lee, Na-Ra; Kwon, Hyun-Mi; Park, Kkothanahreum; Oh, Sangtaek; Jeong, Yong-Joo; Kim, Dong-Eun
Title: Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13
  • Document date: 2010_7_29
    • ID: 1k99yv4i_20
    Snippet: An alternative but not exclusive explanation for the low processivity of nsP13 is that the DNA may not be the proper substrate for nsP13. Despite the fact that the SARS nsP13 helicase is a RNA helicase, little is known about its interaction with RNA. Although we have previously investigated the ATP hydrolysis activity of nsP13 in the presence of ssDNA or ssRNA (25) , more rigorous experiments to monitor its affinities toward DNA or RNA are requir.....
    Document: An alternative but not exclusive explanation for the low processivity of nsP13 is that the DNA may not be the proper substrate for nsP13. Despite the fact that the SARS nsP13 helicase is a RNA helicase, little is known about its interaction with RNA. Although we have previously investigated the ATP hydrolysis activity of nsP13 in the presence of ssDNA or ssRNA (25) , more rigorous experiments to monitor its affinities toward DNA or RNA are required to explain this low processivity. Whether nsP13 has different affinities for various types of DNAs or RNAs might be a determining factor for the processivity of nsP13 in duplex unwinding.

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