Cytoprotective Activity of Glycyrrhizae radix Extract Against Arsenite-induced Cytotoxicity

Kim, Sang Chan; Park, Sook Jahr; Lee, Jong Rok; Seo, Jung Cheol; Yang, Chae Ha; Byun, Sung Hui

Dulbecco's modified Eagle's medium (DMEM) and fetal calf serum (FCS) were obtained from BioWhittaker (Walkersville, MD, USA) and Life Technologies (Gaithersburg, MD, USA). Arsenite, [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] (MTT) and other reagents in the molecular studies were supplied from Sigma Chemical (St. Louis, MO, USA). Licorice water extract was prepared by boiling 600 g of G. radix (Wolsung, Daegu, Korea) in 5 l of water for 3 h, filtering through a 0.2 mm syringe filter (Nalgene, New York, NY, USA), and storing at À20 C until use. The amount of licorice water extract was estimated by the dried weight of lyophilized water extract of G. radix. The yield of lyophilized licorice from G. radix was 13%.