Author: Wan, Gwo-Hwa; Huang, Chung-Guei; Chung, Fen-Fang; Lin, Tzou-Yien; Tsao, Kuo-Chien; Huang, Yhu-Chering
Title: Detection of Common Respiratory Viruses and Mycoplasma pneumoniae in Patient-Occupied Rooms in Pediatric Wards Document date: 2016_4_8
ID: 0dpqgi1r_15
Snippet: RNA and DNA were isolated by using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) and the Qiagen DNA Mini Kit (Qiagen Instruments AG, Hombrechtikon, Switzerland), respectively, according to the manufacturer's directions. Briefly, 500 mL of each sample was extracted, and the nucleic acid was eluted at a final volume of 30 mL. Isolates were stored at À808C until analysis. For negative controls, sterile distilled water was used in place of.....
Document: RNA and DNA were isolated by using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) and the Qiagen DNA Mini Kit (Qiagen Instruments AG, Hombrechtikon, Switzerland), respectively, according to the manufacturer's directions. Briefly, 500 mL of each sample was extracted, and the nucleic acid was eluted at a final volume of 30 mL. Isolates were stored at À808C until analysis. For negative controls, sterile distilled water was used in place of the sample.
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