Selected article for: "ethanol precipitate and high yield rna NEB synthesis kit"
Author: Martin, Baptiste; Coutard, Bruno; Guez, Théo; Paesen, Guido C; Canard, Bruno; Debart, Françoise; Vasseur, Jean-Jacques; Grimes, Jonathan M; Decroly, Etienne
Title: The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure
  • Document date: 2018_9_6
    • ID: 243u68j8_15
    Snippet: Long synthetic RNAs have been produced by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit instructions (NEB), following the manufacturer's instructions. Templates are listed in Supplementary Table S2 . Briefly, 1 g of template was incubated with the T7 RNA polymerase mix and NTPs (40 mM) for 2 h at 37 • C. Sodium acetate pH 5.2 and glycogen (Invitrogen) were added to the mix to 300 mM and 1 g/l, respectively, followed .....
    Document: Long synthetic RNAs have been produced by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit instructions (NEB), following the manufacturer's instructions. Templates are listed in Supplementary Table S2 . Briefly, 1 g of template was incubated with the T7 RNA polymerase mix and NTPs (40 mM) for 2 h at 37 • C. Sodium acetate pH 5.2 and glycogen (Invitrogen) were added to the mix to 300 mM and 1 g/l, respectively, followed by 3 volumes of ethanol to precipitate the RNA. After centrifugation, pellets were washed with 70% ethanol and centrifuged again. Finally, pellets were dried and re-suspended in water.

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