Title: The rubella virus E1 glycoprotein is arrested in a novel post-ER, pre- Golgi compartment Document date: 1992_8_2
ID: 04455ffs_26
Snippet: CHOE1 cells grown on coverslips were infected 2 to 3 d after seeding with VSV ts045 (kindly provided by Dr. William Balch, Scripps Research Institute) such that 50% of the cells were infected at the time of assay. Cells were washed twice with serum-free MEM alpha containing 1/6 the normal amount of sodium bicarbonate plus 10 mM Hepes (pH 7.2) before addition of virus. Virus was allowed to bind to cells for 60 rain at 32"C. The viruscontaining med.....
Document: CHOE1 cells grown on coverslips were infected 2 to 3 d after seeding with VSV ts045 (kindly provided by Dr. William Balch, Scripps Research Institute) such that 50% of the cells were infected at the time of assay. Cells were washed twice with serum-free MEM alpha containing 1/6 the normal amount of sodium bicarbonate plus 10 mM Hepes (pH 7.2) before addition of virus. Virus was allowed to bind to cells for 60 rain at 32"C. The viruscontaining media was then removed and replaced with the same media containing 10% FBS. Ceils were incubated in a 39.5~ water bath for 2.5 h to accumulate G protein in the RER, after which cycloheximide (500 #M) was added, and the cells were transferred to 15~ and/or 320C water baths for various chase periods before assay by indirect immunofluorescence,
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