Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus Document date: 2017_12_2
ID: 4szmu1dh_10
Snippet: The specificity of the ICA kit for other PEDV strains was tested with PEDV-KNU1309 (accession no. KJ451044), PEDV-KNU1303 (accession no. KJ451038) and PEDV-KNU1402 (accession no. KJ451048), which have 99 per cent identity with the viruses identified in the US pig populations. Additionally, the specificity of the ICA kit against PEDV was evaluated for other porcine viruses including TGEV, PRCV and porcine rotavirus obtained from Green Cross Veteri.....
Document: The specificity of the ICA kit for other PEDV strains was tested with PEDV-KNU1309 (accession no. KJ451044), PEDV-KNU1303 (accession no. KJ451038) and PEDV-KNU1402 (accession no. KJ451048), which have 99 per cent identity with the viruses identified in the US pig populations. Additionally, the specificity of the ICA kit against PEDV was evaluated for other porcine viruses including TGEV, PRCV and porcine rotavirus obtained from Green Cross Veterinary Product. For the ICA kit test, the culture supernatant (100 µl) of each virus or cell culture medium as a negative control was transferred into a test tube containing 100 µl of assay diluent (50 mM Tris (pH 8.5), 10 mM NaCl, 0.1 per cent Tween 20, 1 parts per million ProClin 300). The mixture (100 µl) was applied to the sample well of the test kit and the test signal for each test was visualised after 15 minutes.
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