Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain Document date: 1996_7_2
ID: 45x96b5d_34
Snippet: It is evident that the TMD region of Sec12p is sufficient to localize the chimeric proteins to the ER in the RER1 + cells. However, is it solely necessary for the ER localization of Secl2p? The answer is obviously no. Even with the Dap2p TMD, SDSm and SDDm constructs secrete only 30-40% (10-15% if corrected) of a-factor as compared to The amounts of secreted c~-factor in Fig. 4 were quantified and expressed as proportions relative to that secrete.....
Document: It is evident that the TMD region of Sec12p is sufficient to localize the chimeric proteins to the ER in the RER1 + cells. However, is it solely necessary for the ER localization of Secl2p? The answer is obviously no. Even with the Dap2p TMD, SDSm and SDDm constructs secrete only 30-40% (10-15% if corrected) of a-factor as compared to The amounts of secreted c~-factor in Fig. 4 were quantified and expressed as proportions relative to that secreted by DDDm in the wild-type ceils. A value below 5 means that it was below the detection limit. The figures in parentheses are after correction, considering the amount of each chimera estimated by immunoblotting.
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