Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation Document date: 2019_8_6
ID: 5xk3z4ck_67
Snippet: For coimmunoprecipitation, cleared cell extracts lysed in IGEPAL lysis buffer were filled up to a volume of 600 μl with lysis buffer and supplemented with 1 μg precipitating antibody or control IgG. After adding 20 μl protein A/G agarose (Millipore), the samples were incubated for 4 h at 4°C on a rotating wheel. Alternatively, covalently antibody-coupled affinity gels were used for coimmunoprecipitation (anti-Flag M2 affinity gel, A2220, Sigm.....
Document: For coimmunoprecipitation, cleared cell extracts lysed in IGEPAL lysis buffer were filled up to a volume of 600 μl with lysis buffer and supplemented with 1 μg precipitating antibody or control IgG. After adding 20 μl protein A/G agarose (Millipore), the samples were incubated for 4 h at 4°C on a rotating wheel. Alternatively, covalently antibody-coupled affinity gels were used for coimmunoprecipitation (anti-Flag M2 affinity gel, A2220, Sigma-Aldrich;
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