Selected article for: "primer set and specificity sensitivity"
Author: TAKANO, Tomomi; YAMASHITA, Saya; MURATA-OHKUBO, Michiko; SATOH, Kumi; DOKI, Tomoyoshi; HOHDATSU, Tsutomu
Title: Prevalence of canine coronavirus (CCoV) in dog in Japan: detection of CCoV RNA and retrospective serological analysis
  • Document date: 2015_10_11
    • ID: 2kg967bh_9
    Snippet: We herein detected CCoV gene in dogs in Japan. CCoV gene detection in dogs in Japan has been performed, similarly to our study. The CCoV gene-positive rates in our study and reported by Bandai et al. [1] and Soma et al. [12] were 26.7, 16.0 and 50.5%, respectively, showing variation among the studies, and this may have been influenced by differences in the target gene. Moreover, the age and maintenance environment of the dogs may also have influe.....
    Document: We herein detected CCoV gene in dogs in Japan. CCoV gene detection in dogs in Japan has been performed, similarly to our study. The CCoV gene-positive rates in our study and reported by Bandai et al. [1] and Soma et al. [12] were 26.7, 16.0 and 50.5%, respectively, showing variation among the studies, and this may have been influenced by differences in the target gene. Moreover, the age and maintenance environment of the dogs may also have influenced the results of RT-PCR. It is desirable for an epidemiological survey of CCoV infection to be performed specifying a primer set with the highest sensitivity and specificity.

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