Selected article for: "cell growth and fetal bovine serum"
Author: Jung, Juwon; Baek, Jin Ah; Seol, Hye Won; Choi, Young Min
Title: Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions
  • Document date: 2016_3_23
    • ID: 20xe4vm6_6
    Snippet: The hESC line used in the present study was SNUhES32 and 10% fetal bovine serum (FBS; Hyclone) using an incubator (37°C, 5% CO 2 ) for 3 days. To inactivate hAFCs, cell growth was suppressed by treatment with mitomycin C (Sigma) for 2.5 h, and the cells were then transferred to a CELLStart-coated 35-mm culture dish at a density of 2.0×10 4 cells/cm 2 ......
    Document: The hESC line used in the present study was SNUhES32 and 10% fetal bovine serum (FBS; Hyclone) using an incubator (37°C, 5% CO 2 ) for 3 days. To inactivate hAFCs, cell growth was suppressed by treatment with mitomycin C (Sigma) for 2.5 h, and the cells were then transferred to a CELLStart-coated 35-mm culture dish at a density of 2.0×10 4 cells/cm 2 .

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