Author: Baschera, Melinda; Cernela, Nicole; Stevens, Marc J.A.; Liljander, Anne; Jores, Jörg; Corman, Victor Max; Nüesch-Inderbinen, Magdalena; Stephan, Roger
Title: Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels Document date: 2019_3_7
ID: 70exgy3h_12
Snippet: Each fecal sample was enriched at a 1:10 ratio in Enterobacteriaceae enrichment (EE) broth (Becton, Dickinson, Heidelberg, Germany) for 24 h at 37°C. One loopful of each of the enrichment cultures was cultured on sheep blood agar (Difco™ Columbia Blood Agar Base EH; Becton Dickinson AG, Allschwil, Switzerland) using the streak plate technique. The resulting colonies were washed off with 2 ml 0.85% NaCl and DNA was extracted by a standard lysis.....
Document: Each fecal sample was enriched at a 1:10 ratio in Enterobacteriaceae enrichment (EE) broth (Becton, Dickinson, Heidelberg, Germany) for 24 h at 37°C. One loopful of each of the enrichment cultures was cultured on sheep blood agar (Difco™ Columbia Blood Agar Base EH; Becton Dickinson AG, Allschwil, Switzerland) using the streak plate technique. The resulting colonies were washed off with 2 ml 0.85% NaCl and DNA was extracted by a standard lysis protocol. Screening for stx1 and stx2 genes was performed by real-time PCR (LightCycler R 2.0 Instrument, Roche Diagnostics Corporation, Indianapolis, IN, USA) using the QuantiFast Multiplex PCR Kit (Qiagen, Hombrechtikon, Switzerland) according to the guidelines of the European Union Reference Laboratory (EURL) [25] .
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