Author: Baschera, Melinda; Cernela, Nicole; Stevens, Marc J.A.; Liljander, Anne; Jores, Jörg; Corman, Victor Max; Nüesch-Inderbinen, Magdalena; Stephan, Roger
Title: Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels Document date: 2019_3_7
ID: 70exgy3h_19
Snippet: Genomic DNA libraries were prepared using the Nextera™ DNA Flex Library Preparation Kit following the manufacturer's protocol (Illumina, San Diego, CA, USA). Libraries were loaded on a 300-cycle, 2 × 150 bp paired-end read cartridge (Illumina MiniSeq High Output Reagent Kit) and run on a MiniSeq Illumina sequencer (Illumina). The Spades assembler v3.12 [27] was used to assemble the reads with using the option "-careful". The spades output was .....
Document: Genomic DNA libraries were prepared using the Nextera™ DNA Flex Library Preparation Kit following the manufacturer's protocol (Illumina, San Diego, CA, USA). Libraries were loaded on a 300-cycle, 2 × 150 bp paired-end read cartridge (Illumina MiniSeq High Output Reagent Kit) and run on a MiniSeq Illumina sequencer (Illumina). The Spades assembler v3.12 [27] was used to assemble the reads with using the option "-careful". The spades output was filtered for size > 1000 bp and coverage > 25-fold resulting in the final assemblies that were submitted to NCBI GenBank.
Search related documents:
Co phrase search for related documents- genomic dna and manufacturer protocol: 1
- genomic dna and pair end: 1
Co phrase search for related documents, hyperlinks ordered by date